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European BioPharmaceutical Review
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Recombinant DNA technologies are widely used in the drug discovery process, not only to produce novel, engineered proteins, but also to generate proteins in the quantities required for downstream research. Purifying these proteins remains a bottleneck. This article looks at a novel method to rapidly isolate structurally and functionally intact histidine-tagged recombinant proteins using superparamagnetic beads.
Recombinant DNA (rDNA) technologies are a cornerstone of the biotechnology industry and have revolutionised approaches to the discovery of new medicines. Pioneered by scientists such as Paul Berg, Stanley Cohen and Herbert Boyer in the early 1970s, rDNA methodologies allow scientists to 'cut and paste' DNA to engineer novel proteins, and to transfer DNA from one organism to another. They also provide the means to generate proteins in the quantities required for downstream studies in drug discovery - from target identification, target validation and screening of compound libraries, to ADME-Tox studies.
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Clinical Trials in CNS
25-26 November 2008, Copthorne Tara Hotel, London, UK
SMi present their 7th annual conference… Clinical Trials in CNS 25th to 26th November 2008, Copthorne Tara Hotel, London, UK
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