|
|
|
| home > ebr > spring 2003 > surface plasmon resonance applied to the high-throughput measurement of molecular binding |
|
|
 PUBLICATIONS |
European Biopharmaceutical Review
|
Basic Principles
Surface plasmon resonance (SPR) is an established optical technique for the detection of molecular binding, based on the generation of surface plasmons in a thin metal film (typically gold) that supports the binding chemistry. Surface plasmons are collective oscillations of free electrons constrained in the metal film.
These electrons are excited resonantly by a light field incident from a highly refractive index prism as shown in Figure 1. The angle of incidence q over which this resonant excitation occurs is relatively narrow, and is characterised by a reduction in the intensity of the reflected light which has a minimum at the resonant angle of incidence qr. The phase of the reflected light also varies nearly linearly with respect to q in this region.
The value of qr is a sensitive to the refractive index of the medium that resides within a few nanometres of the metal film. Small variations in the refractive index, due to the binding of a molecule to the film, may therefore be detected as a variation of this angle. In practice a thin layer of specific binding chemistry is deposited on the metal. Binding is then induced by flowing a fluid containing the target molecules over this composite surface.
|
Read full article from PDF >>
|
|
 |
|
| Rate this article |
You must be a member of the site to make a vote. |
|
Average rating: |
0 |
| | | | | |
|
|
 |
Industry Events |
 |
BIO-Europe Spring® 2012 — 6th International Partnering Conference
19-21 March 2012, Amsterdam RAI Convention Center, Amsterdam, The Netherlands
BIO-Europe Spring is the springtime counterpart to EBD Group’s flagship conference, BIO-Europe, and continues the tradition of providing life science companies with high caliber partnering opportunities. Featuring EBD Group’s sophisticated, web-based partnering system, partneringONE®, the event enables delegates to efficiently identify, meet and get partnerships started with companies across the life science value chain, from large biotech and pharma companies to financiers and innovative start-ups.
More info >> |
|
 |
News and Press Releases |
 |
Pantec Biosolutions Showcases P.L.E.A.S.E.® Professional at the IMCAS Annual Congress
Pantec Biosolutions AG, the developer and manufacturer of P.L.E.A.S.E.® Professional, a state of the art portable laser device that sets new standards for safety, precision and versatility, today announced that it will present its recently launched P.L.E.A.S.E.® Professional laser device at the International Master Course on Aging Skin (IMCAS) from 26th-29th January 2012 in Paris, France.
More info >> |
|
|
