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| home > ebr > winter 2002 > truncate rna preparation in crna labelling reactions without sacrificing microarray performance? |
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European BioPharmaceutical Review
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| When preparing cRNA samples for microarray analysis, researchers must choose between mRNA and total RNA as the starting material for labelling. The method of choice for high quality mRNA preparation typically involves total RNA extraction followed by an additional step of mRNA purification. While this method generally yields high quality substrates for microarray target preparation, its major drawback is the addition of an extra step to the preparation process which increases the amount of product lost by approximately 30% and also increases the potential for sample degradation, both of which result in lower amplified cRNA yields and decreased microarray sensitivity. In light of these issues, microarray researchers have recently developed protocols to improve the feasibility of using total RNA directly in the labelling reaction. |
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