Determination of Absorptivity Constants for Biopharmaceutical Protein Products by Amino Acid Analysis

By John C Anders, K Chad Bastian and Glenn E Petrie, Research Associates at AAI International John C Anders received his PhD in Biochemistry and Molecular Biology from the University of Florida in 1990. He completed his postdoctoral work in the Department of Immunology at the Walter Reed Army Institute of Research, Washington DC on development of a malaria vaccine. Subsequent military service at the Medical Research Institute of Chemical Defense focused on bringing biotechnological innovations to the treatment and amelioration of the toxic effects of various chemical warfare agents.
Dr Anders now serves as a Senior Research Associate in the Biotechnology Laboratory of AAI International. His current work focuses on various aspects of protein chemistry upon analysis of biopharmaceuticals in a cGMP laboratory environment.

K Chad Bastian received his PhD in Civil Engineering from Purdue University in 1996 and completed a postdoctoral appointment with the US Geological Survey through the University of Kansas Center for Research, Inc.

Dr Bastian is currently employed by AAI International focusing on applications of HPLC/MS and tandem mass spectrometry to pharmaceutical and biopharmaceutical analysis in a GMP environment.

Glenn Petrie received his PhD in Biochemistry from the University of Illinois in September 1983. He began his career at Allied Signal, Research & Technology, where he designed, staffed and managed a $1.5 million speciality analytical laboratory to support a diverse corporate research laboratory.

He is currently employed in the Analytical Division of AAI International, where he provides technical supervision to a staff of PhD, MS and BS scientists. He works closely with clients to define and manage their method development and validation projects.