samedan logo
 
 
spacer
home > news > detailed info
INDUSTRY NEWS AND PRESS RELEASES

Cancer immunotherapy with the help of CAR-T cells

PlasmidFactory GmbH & Co. KG

Sleeping Beauty Minicircles enable production of CAR-T cells

Bielefeld, October 2017 – PlasmidFactory, the leading contract manufacturer for plasmid and minicircle (MC) DNA, once again highlights the cooperation with the University Hospital of Würzburg and the MD-CEL – Helmholtz Innovation Lab at the Max Delbrück Centre (MDC) Berlin in the area of cancer immunotherapy. This cancer therapy uses CAR-T cells that recognise and destroy cancer cells. The patient’s own immune cells are removed, genetically modified, and then re-introduced into the body. In conventional immunotherapies, which have shown remarkable efficacy in clinical trials, T cells are modified by retroviral vectors. However, the potential for insertional mutagenesis and genotoxicity of viral vectors poses a safety risk, and the cost and regulatory requirements will stand in the way of a fast and broad clinical application. In late August, the first CAR-T cell therapy for acute lymphoblastic leukaemia (ALL) was approved in the US.

Unlike this therapy, the genetically modified CAR-T cells are produced in a virus-free method based on Sleeping Beauty Minicircle in cooperation with PlasmidFactory. The Sleeping Beauty (SB) system includes a transposon, which is used in gene and cell therapy, in order to introduce therapeutic sequences into the genome of patient cells. Through chromosomal integration, these sequences are firmly anchored there and used, for example, to complement the genetic characteristics or to make the cell identifiable.

“In collaboration with the University Hospital of Würzburg and the MD-CEL, we have created an opportunity for the MC-based SB transposition of chimeric antigen receptor (CAR) transgenes using this innovative system. Because of the rapid production of CAR-T cells for clinical use, this allows an improved transposition compared with conventional plasmids,” explains PlasmidFactory’s Managing Director, Dr Martin Schleef.

Minicircles are small, circular DNA molecules that contain almost only the desired expression cassette. Because of the missing bacterial backbone, minicircles have now become established as alternative vectors for the transfection of eukaryotic cells. The modification of cells based on minicircle technology is combined with a transposon system. By including a transposition cassette on a minicircle, higher transposition rates of the desired gene segment can be achieved than with conventional vectors. Such minicircle-based therapies also promise a lower safety risk.

About PlasmidFactory
PlasmidFactory GmbH & Co. KG is a biopharmaceutical company founded in 2000 in Bielefeld. It has since developed into an international company. In addition to custom manufacturing of plasmid and minicircle DNA, PlasmidFactory focuses its research and development efforts on its core competencies in the production, analysis, application, and storage of plasmid and minicircle DNA.

Minicircle DNA contains effectively only the gene of interest (GOI). Unnecessary sequences used only for the production process of plasmids such as antibiotic resistance genes or the origin of replication (ori) were almost completely removed. The result is a safe and highly-effective vector system. It already meets the future regulatory requirements for gene therapy and vaccination.

Media Contact
Tatjana Buchholz
Marketing Manager, M.Sc.
T.: +49 521 299 7350
E.: info@plasmidfactory.com
Web: PlasmidFactory.com
phone +49 (0) 521 299 735 0
email info@plasmidfactory.com
web www.plasmidfactory.com
email Meisenstraße 96, D-33607 Bielefeld, Germany
 
Print this page
Send to a friend
   
spacer
News and Press Releases

Digital Manufacturing is a top priority for Life Sciences according to Zenith survey

Zenith Technologies, a world leader in delivering GMP manufacturing software solutions to the life sciences industry, recently conducted an industry survey that suggests the digital manufacturing is a top priority.
More info >>


White Papers

Successfully managing the unique demands of cell therapy supply chains

PCI Pharma Services

Cell therapy professionals joined a specialist webinar by industry experts from PCI Clinical Services and TrakCel, addressing the unique complexity of an autologous therapy supply chain. Hosted by European Pharmaceutical Manufacturer magazine, the webinar was delivered by Rachel Griffiths, Associate Director, Technical Services, PCI Clinincal Services, and Dr. Matthew Lakelin, Vice President, Scientific Affairs and Business Development, TrakCel . Here, we present the white paper from that webinar event.
More info >>

Industry Events

SMi Presents the 11th Adaptive Designs in Clinical Trials Conference 2019

1-2 April 2019, Holiday Inn Kensington Forum, London

SMi Group are proud to announce the 11th annual Adaptive Designs in Clinical Trials conference and exhibition will return to London from the 1st – 2nd April 2019. With the growing popularity in use and acceptance of adaptive designs in clinical trials - demonstrated most recently with the new FDA draft guidance on adaptive designs – there is a pressing need for a forum which explores this increasingly normalized medical methodology.
More info >>

 
©2000-2011 Samedan Ltd.
Add to favourites

Print this page

Send to a friend
Privacy statement