|
|
|
| home > white papers > Medpace Reference Laboratories establishes state of the art Flow Cytometry techniques for flexible approaches to clinical trials across multiple therapeutic areas. - Medpace |
|
|
 WHITE PAPERS |
|
Medpace
|
|
Medpace Reference Laboratories establishes state of the art Flow Cytometry techniques for flexible approaches to clinical trials across multiple therapeutic areas.
|
|
Cytometry is the process of measuring the properties of individual cells. These properties may include gene or protein expression, chemical properties, deoxyribonucleic acid (DNA) content, and various cellular functions. The earliest methods of cytometry relied upon light microscopy for the classification and observation of cells and cellular components. Microscopy permitted direct visual observation of cells for the first time, leading to the classification of cells by morphology and insight into cellular functions. However, the time required for microscopic analysis constrains the number of samples or number of cells in each sample that can be examined. Therefore, the utility of microscopy for analysis of rare cells or in situations where sample throughput is a priority is limited. Flow cytometry was developed largely to improve upon these limitations.
Flow cytometry depends upon the ability to pass cells single file in a solution through the path of one or more laser beams. Flowing cells through the path of the laser permits the analysis of thousands of cells per second, but requires that the cells in the sample are in a single-cell, liquid suspension. Therefore, solid tissue samples require dissociation through mechanical or enzymatic processing before analysis. A wide variety of information about the cell can be determined depending upon how the cell interacts with the light from the laser. Detectors that measure the manner in which the cell scatters light can provide information about the size of the cell as well as complexity due to presence of intracellular granules and irregularities in the shape of the cell membrane. On the basis of light scatter properties alone, three distinct populations of leukocytes can be resolved in the peripheral blood: lymphocytes, monocytes and granulocytes. However, the power and flexibility of flow cytometry is fully realized when fluorescent probes that can be excited by the light from the laser are incorporated.
|
|
|
|
|
|
|
 |
News and Press Releases |
 |
Janssen Presents Updated Data Demonstrating Improved Outcomes from the Use of Niraparib in Combination with Abiraterone Acetate Plus Prednisone as a First-Line Therapy in Patients with BRCA-Positive Metastatic Castration-Resistant Prostate Cancer
BEERSE, BELGIUM, 16 February 2023 – The Janssen Pharmaceutical
Companies of Johnson & Johnson today announced updated results from
the Phase 3 MAGNITUDE study evaluating the investigational use of
niraparib, a highly selective poly (ADP-ribose) polymerase (PARP)
inhibitor, in combination with abiraterone acetate plus prednisone (AAP)
in patients with metastatic castration-resistant prostate cancer
(mCRPC) with or without specific homologous recombination repair (HRR)
gene alterations, including BRCA mutations.1 Results will be
featured in a Rapid Abstract Session (Abstract #170) at the American
Society of Clinical Oncology’s Genitourinary (ASCO GU) Cancers Symposium
2023, taking place February 16-18.1
More info >> |
|
 |
White Papers |
 |
|
Orthogonal Approaches for the Analysis of Protein Sequence
Ludger Ltd
One of the most important classes of biopharmaceuticals is monoclonal antibodies. They are expressed from living cells, and are consequently subject to complex biochemical pathways. Not all of these routes are fully understood, and many are sensitive to the subtlest of environmental changes. Changes such as these can affect the final biopharmaceutical sequence, structure and post-translational modifications, not to mention any changes that may occur during further purification. This often means that the final product from one batch may be different from the last in another batch. Each batch is a complicated mix of similar molecules, and analysis of the degree of batch-to-batch variation is therefore very important to the establishment of the drug's safety for medicinal use and patient administration.
More info >> |
|
 |
Industry Events |
 |
The Pharmaceutical Manufacturing and Packaging Congress 2023 (PHARMAP 2023)
12-13 June 2023, Hilton Geneva Hotel & Conference Centre, Geneva, Switzerland
The Pharmaceutical Manufacturing and Packaging Congress 2023 (PHARMAP 2023) gathers top-level management from pharmaceutical
companies of manufacturing, primary and secondary packaging, purchasing,
and supply chain and procurement sectors, CMOs and CDMOs leaders, heads
from government and institution on 12-13, June, 2023 in Geneva,
Switzerland.
More info >> |
|
|
|
